The determination of glucose and HbA1c is essential to diagnose diabetes mellitus. HbA1c is a form of hemoglobin chemically linked to glucose. Glucose is in serum or plasma and HbA1c is inside of RBCs. Glucose can be determined directly from serum and plasma.
On the other hand, HbA1c is determined from lysing solution after RBCs are lysed. Clinical chemistry analyzers equipped with lysing function enable hemolysis of RBCs and the simultaneous determination of glucose and HbA1c.
Hb and HbA1c can be released from erythrocytes by hemolysis treatment. Latex particles are immobilized with HbA1c in the sample. Immobilized latex particles by Hb and HbA1c react with mouse anti-human HbA1c monoclonal antibody and goat anti-mouse IgG polyclonal antibody. The agglutination of latex, HbA1c and two types of antibodies is proportional to the percentage of HbA1c. Therefore, the percentage of HbA1C can be determined.
Clinical chemistry analyzer, the CA-800, has the function to lyse RBCs on board. RBCs are lysed in cuvette with lysing solution.
This lysed sample is used for HbA1c determination. This enables the simultaneous determination of Glucose and HbA1c from one blood collecting tube with anticoagulant.
The combination between Furuno CA-800, Centronic HbA1c reagent and calibrator gets NGSP certificate of traceability every year.
Centronic HbA1c reagent shows good correlation to enzymatic methodology, too.
Product Name | Product No. | FURUNO Analyzer | Configuration | ||
R1 | R2 | Other | |||
Glucose HK fluid 5+1 | GF02917084-F3 | CA-270 | 3 × 67 mL | 3 × 17 mL | - |
GF02917084-F | 6 × 67 mL | 6 × 17 mL | - | ||
GF02917084-F4 | 4 × 20 mL | 4 × 6 mL | - | ||
GF02917084-F8 | 8 × 20 mL | 8 × 6 mL | - | ||
HbA1c | HF04917040-F | CA-270 | 1 × 30 mL | 12 × 20 mL | Lysing solution 2 × 60mL |
HbA1c Calibration set | CCALHbA1cT | CA-270 | 4 levels × 0.5 mL | ||
HbA1c Control low | CNORMHbA1c | 1 levels × 0.5 mL | |||
HbA1c Control high | CPATHHbA1c | 1 levels × 0.5 mL | |||
Reagents are manufactured by Centronic GmbH certified according to ISO13485.
